ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (5): 752-759.doi: 10.11843/j.issn.0366-6964.2015.05.010

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Identification and Bioinformatic Analysis on Melanocortin-1 Receptor Gene(MC1R)of American Mink(Neovison vison

SONG Xing-chao,XU Chao,YUE Zhi-gang,WANG Lei,CONG Bo,LIU Lin-ling,YANG Fu-he*   

  1. (State Key Laboratory of Special Economic Animal Molecular Biology,Institute of Special Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Changchun 130112,China)
  • Received:2014-07-11 Online:2015-05-23 Published:2015-05-23

Abstract:

In order to explore the sequence structure of MC1R gene and its regulating mechanism on coat color of American mink.One pair special primer was designed according to the MC1R gene of Mustela lutreola(Accession No.:AB189820) published in GenBank.The complete coding sequence(1 324 bp) of MC1R gene were obtained by PCR and cloning technique from the blood total DNA of Neovison vison,then the structural characteristics of nucleotide and amino acid sequence were predicted and analyzed by bioinformatics method.The results showed that the sequence of American mink MC1R gene consisted of one single exon,partial of 5′UTR(188 bp) and 3′UTR(182 bp),and it contained an open reading frame of 954 bp,which encoded 317 amino acids.The estimated molecular weight of MC1R protein was 34.49 ku,with a isoelectric point of 8.97 and 36.63 in instability index,belonging to the weakly stable basic protein.Further structure prediction indicated that the MC1R protein had one simple domain,7 transmembrane domains and no signal peptide at N-terminal,it was located on plasma membrane.The similarity comparison and phylogenetic tree indicated that the evolution distance of American mink MC1R was the most homogeneous to Mustela lutreola (95%) belong to Mustelidae family.Identification and sequence analysis of American mink MC1R gene would lay the bioinformatics foundation for further investigating correlations between polymorphisms of MC1R gene and coat color phenotype in mink.

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